Stability of catechins in green tea nutraceutical products: Application of solid phase extraction–thin layer chromatography densitometry
Author: Heba-Alla H. Abd-ElSalam and Medhat A. Al-Ghobashy and Hala E. Zaazaa and Mohamed A. Ibrahim
Epigallocatechin gallate (EGCG) is a powerful antioxidant and commonly used nutraceutical. Accelerated stability of EGCG in tablet formulations was investigated. LLE and SPE were employed for sample clean-up and enrichment of EGCG over caffeine. Samples were analysed after spiking with fixed concentration of gallic acid (GA), in order to verify reproducibility of analysis. A TLC–densitometric assay was developed and validated for determination of % loss EGCG. EGCG, GA and caffeine were resolved with Rf values 0.54, 0.69 and 0.80, respectively. LC–MS/MS was used to verify identity and purity of the EGCG band. Determination was carried out over a concentration range of 0.50–5.00 μg/band and 0.20–2.40 μg/band for GA and caffeine, respectively. Results showed significant reduction in EGCG content after one, three and six months: 24.00%, 28.00% and 52.00% respectively. Results continue to demonstrate that stability of nutraceutical products should be investigated in-depth using industry-oriented protocols before granting marketing authorisation.